Validation And Uncertainty Estimation Of A Method By High Performance Liquid Chromatography For The Analysis Of Lycopene And β-carotene In Tomato Pulp [validação E Estimativa Da Incerteza De Método Para Análise De Licopeno E B-caroteno Em Polpa De Tomate Por Cromatografia Líquida De Alta Eficiência]

摘要

A method to quantify lycopene and β-carotene in freeze dried tomato pulp by high performance liquid chromatography (HLPC) was validated according to the criteria of selectivity, sensitivity, precision and accuracy, and uncertainty estimation of measurement was determined with data obtained in the validation. The validated method presented is selective in terms of analysis, and it had a good precision and accuracy. Detection limit for lycopene and β-carotene was 4.2 and 0.23 mg 100 g-1, respectively. The estimation of expanded uncertainty (K = 2) for lycopene was 104 ± 21 mg 100 g-1 and for β-carotene was 6.4 ± 1.5 mg 100 g-1.